Molecular Characterization and Biofilm Formation of Escherichia coli from Vegetables

Escherichia coli can cause diarrheal and extraintestinal illnesses in humans. Diarrheagenic E. be transmit to human through consumption of contaminated food, including vegetables. Biofilm produced by during food processing plays a role development foodborne illnesses. Vegetables have often been involved infections. A total 40 isolates from vegetables were tested determine biofilm formation at 12°C, 25°C 37°C the crystal violet MTT assays. All performed for production curli fimbriae cellulose associated with on Congo red agar. 37°C, 12°C was detected 87.5%, 70% isolates, respectively. among using assays showed statistically significant difference between as well (p < 0.05). However, no 25 > 0.05) obtained. Three different morhotypes (bdar, pdar saw) identified based expression cellulose. The incidence bdar morhotype 27.5% 50% Prevalence morphotype At 25°C, only one isolate (2.5%) saw morphotype. expressed or cellulose, three which non-biofilm producer assay. This study demonstrated that presence forming E.coli may risk health safety.